diff --git a/newsfragments/3389.changed.md b/newsfragments/3389.changed.md
new file mode 100644
index 00000000..2f695c7f
--- /dev/null
+++ b/newsfragments/3389.changed.md
@@ -0,0 +1 @@
+`PyIterator::from_object` and `PyByteArray::from` now take a single argument of type `&PyAny`, rather than two arguments, `Python` and `AsPyPointer`
diff --git a/src/types/any.rs b/src/types/any.rs
index 0192bfa3..2ff65275 100644
--- a/src/types/any.rs
+++ b/src/types/any.rs
@@ -818,7 +818,7 @@ impl PyAny {
     /// This is typically a new iterator but if the argument is an iterator,
     /// this returns itself.
     pub fn iter(&self) -> PyResult<&PyIterator> {
-        PyIterator::from_object(self.py(), self)
+        PyIterator::from_object(self)
     }
 
     /// Returns the Python type object for this object's type.
diff --git a/src/types/bytearray.rs b/src/types/bytearray.rs
index 9c03ddec..1ec244a4 100644
--- a/src/types/bytearray.rs
+++ b/src/types/bytearray.rs
@@ -64,11 +64,11 @@ impl PyByteArray {
 
     /// Creates a new Python `bytearray` object from another Python object that
     /// implements the buffer protocol.
-    pub fn from<'p, I>(py: Python<'p>, src: &'p I) -> PyResult<&'p PyByteArray>
-    where
-        I: AsPyPointer,
-    {
-        unsafe { py.from_owned_ptr_or_err(ffi::PyByteArray_FromObject(src.as_ptr())) }
+    pub fn from(src: &PyAny) -> PyResult<&PyByteArray> {
+        unsafe {
+            src.py()
+                .from_owned_ptr_or_err(ffi::PyByteArray_FromObject(src.as_ptr()))
+        }
     }
 
     /// Gets the length of the bytearray.
@@ -305,7 +305,7 @@ mod tests {
             let bytearray = PyByteArray::new(py, src);
 
             let ba: PyObject = bytearray.into();
-            let bytearray = PyByteArray::from(py, &ba).unwrap();
+            let bytearray = PyByteArray::from(ba.as_ref(py)).unwrap();
 
             assert_eq!(src, unsafe { bytearray.as_bytes() });
         });
@@ -314,7 +314,7 @@ mod tests {
     #[test]
     fn test_from_err() {
         Python::with_gil(|py| {
-            if let Err(err) = PyByteArray::from(py, &py.None()) {
+            if let Err(err) = PyByteArray::from(py.None().as_ref(py)) {
                 assert!(err.is_instance_of::<exceptions::PyTypeError>(py));
             } else {
                 panic!("error");
diff --git a/src/types/frozenset.rs b/src/types/frozenset.rs
index d27e87ef..bbcd7102 100644
--- a/src/types/frozenset.rs
+++ b/src/types/frozenset.rs
@@ -124,7 +124,7 @@ mod impl_ {
 
         fn into_iter(self) -> Self::IntoIter {
             PyFrozenSetIterator {
-                it: PyIterator::from_object(self.py(), self).unwrap(),
+                it: PyIterator::from_object(self).unwrap(),
             }
         }
     }
diff --git a/src/types/iterator.rs b/src/types/iterator.rs
index 6bacb438..c70c08f6 100644
--- a/src/types/iterator.rs
+++ b/src/types/iterator.rs
@@ -30,11 +30,11 @@ impl PyIterator {
     /// Constructs a `PyIterator` from a Python iterable object.
     ///
     /// Equivalent to Python's built-in `iter` function.
-    pub fn from_object<'p, T>(py: Python<'p>, obj: &T) -> PyResult<&'p PyIterator>
-    where
-        T: AsPyPointer,
-    {
-        unsafe { py.from_owned_ptr_or_err(ffi::PyObject_GetIter(obj.as_ptr())) }
+    pub fn from_object(obj: &PyAny) -> PyResult<&PyIterator> {
+        unsafe {
+            obj.py()
+                .from_owned_ptr_or_err(ffi::PyObject_GetIter(obj.as_ptr()))
+        }
     }
 }
 
@@ -209,7 +209,7 @@ def fibonacci(target):
     fn int_not_iterable() {
         Python::with_gil(|py| {
             let x = 5.to_object(py);
-            let err = PyIterator::from_object(py, &x).unwrap_err();
+            let err = PyIterator::from_object(x.as_ref(py)).unwrap_err();
 
             assert!(err.is_instance_of::<PyTypeError>(py));
         });
diff --git a/src/types/set.rs b/src/types/set.rs
index e10979c2..68b89d65 100644
--- a/src/types/set.rs
+++ b/src/types/set.rs
@@ -149,7 +149,7 @@ mod impl_ {
         /// If PyO3 detects that the set is mutated during iteration, it will panic.
         fn into_iter(self) -> Self::IntoIter {
             PySetIterator {
-                it: PyIterator::from_object(self.py(), self).unwrap(),
+                it: PyIterator::from_object(self).unwrap(),
             }
         }
     }