diff --git a/src/conversion.rs b/src/conversion.rs
index aec15a05..50b5a698 100644
--- a/src/conversion.rs
+++ b/src/conversion.rs
@@ -165,7 +165,7 @@ where
 /// Extract reference to instance from `PyObject`
 impl<'a, T> FromPyObject<'a> for &'a T
 where
-    T: PyTryFrom,
+    T: PyTryFrom<'a>,
 {
     #[inline]
     default fn extract(ob: &'a PyObjectRef) -> PyResult<&'a T> {
@@ -176,7 +176,7 @@ where
 /// Extract mutable reference to instance from `PyObject`
 impl<'a, T> FromPyObject<'a> for &'a mut T
 where
-    T: PyTryFrom,
+    T: PyTryFrom<'a>,
 {
     #[inline]
     default fn extract(ob: &'a PyObjectRef) -> PyResult<&'a mut T> {
@@ -221,33 +221,35 @@ pub trait PyTryInto<T>: Sized {
 
 /// Trait implemented by Python object types that allow a checked downcast.
 /// This trait is similar to `std::convert::TryFrom`
-pub trait PyTryFrom: Sized {
+pub trait PyTryFrom<'v>: Sized {
     /// Cast from a concrete Python object type to PyObject.
-    fn try_from(value: &PyObjectRef) -> Result<&Self, PyDowncastError>;
+    fn try_from<V: Into<&'v PyObjectRef>>(value: V) -> Result<&'v Self, PyDowncastError>;
 
     /// Cast from a concrete Python object type to PyObject. With exact type check.
-    fn try_from_exact(value: &PyObjectRef) -> Result<&Self, PyDowncastError>;
+    fn try_from_exact<V: Into<&'v PyObjectRef>>(value: V) -> Result<&'v Self, PyDowncastError>;
 
     /// Cast from a concrete Python object type to PyObject.
-    fn try_from_mut(value: &PyObjectRef) -> Result<&mut Self, PyDowncastError>;
+    fn try_from_mut<V: Into<&'v PyObjectRef>>(value: V) -> Result<&'v mut Self, PyDowncastError>;
 
     /// Cast from a concrete Python object type to PyObject. With exact type check.
-    fn try_from_mut_exact(value: &PyObjectRef) -> Result<&mut Self, PyDowncastError>;
+    fn try_from_mut_exact<V: Into<&'v PyObjectRef>>(
+        value: V,
+    ) -> Result<&'v mut Self, PyDowncastError>;
 
     /// Cast a PyObjectRef to a specific type of PyObject. The caller must
     /// have already verified the reference is for this type.
-    unsafe fn try_from_unchecked(value: &PyObjectRef) -> &Self;
+    unsafe fn try_from_unchecked<V: Into<&'v PyObjectRef>>(value: V) -> &'v Self;
 
     /// Cast a PyObjectRef to a specific type of PyObject. The caller must
     /// have already verified the reference is for this type.
     #[allow(clippy::mut_from_ref)]
-    unsafe fn try_from_mut_unchecked(value: &PyObjectRef) -> &mut Self;
+    unsafe fn try_from_mut_unchecked<V: Into<&'v PyObjectRef>>(value: V) -> &'v mut Self;
 }
 
 // TryFrom implies TryInto
 impl<U> PyTryInto<U> for PyObjectRef
 where
-    U: PyTryFrom,
+    U: for<'v> PyTryFrom<'v>,
 {
     type Error = PyDowncastError;
 
@@ -265,11 +267,12 @@ where
     }
 }
 
-impl<T> PyTryFrom for T
+impl<'v, T> PyTryFrom<'v> for T
 where
     T: PyTypeInfo,
 {
-    fn try_from(value: &PyObjectRef) -> Result<&T, PyDowncastError> {
+    fn try_from<V: Into<&'v PyObjectRef>>(value: V) -> Result<&'v T, PyDowncastError> {
+        let value = value.into();
         unsafe {
             if T::is_instance(value) {
                 Ok(PyTryFrom::try_from_unchecked(value))
@@ -279,7 +282,8 @@ where
         }
     }
 
-    fn try_from_exact(value: &PyObjectRef) -> Result<&T, PyDowncastError> {
+    fn try_from_exact<V: Into<&'v PyObjectRef>>(value: V) -> Result<&'v T, PyDowncastError> {
+        let value = value.into();
         unsafe {
             if T::is_exact_instance(value) {
                 Ok(PyTryFrom::try_from_unchecked(value))
@@ -289,7 +293,8 @@ where
         }
     }
 
-    fn try_from_mut(value: &PyObjectRef) -> Result<&mut T, PyDowncastError> {
+    fn try_from_mut<V: Into<&'v PyObjectRef>>(value: V) -> Result<&'v mut T, PyDowncastError> {
+        let value = value.into();
         unsafe {
             if T::is_instance(value) {
                 Ok(PyTryFrom::try_from_mut_unchecked(value))
@@ -299,7 +304,10 @@ where
         }
     }
 
-    fn try_from_mut_exact(value: &PyObjectRef) -> Result<&mut T, PyDowncastError> {
+    fn try_from_mut_exact<V: Into<&'v PyObjectRef>>(
+        value: V,
+    ) -> Result<&'v mut T, PyDowncastError> {
+        let value = value.into();
         unsafe {
             if T::is_exact_instance(value) {
                 Ok(PyTryFrom::try_from_mut_unchecked(value))
@@ -310,7 +318,8 @@ where
     }
 
     #[inline]
-    unsafe fn try_from_unchecked(value: &PyObjectRef) -> &T {
+    unsafe fn try_from_unchecked<V: Into<&'v PyObjectRef>>(value: V) -> &'v T {
+        let value = value.into();
         let ptr = if T::OFFSET == 0 {
             value as *const _ as *const u8 as *const T
         } else {
@@ -320,7 +329,8 @@ where
     }
 
     #[inline]
-    unsafe fn try_from_mut_unchecked(value: &PyObjectRef) -> &mut T {
+    unsafe fn try_from_mut_unchecked<V: Into<&'v PyObjectRef>>(value: V) -> &'v mut T {
+        let value = value.into();
         let ptr = if T::OFFSET == 0 {
             value as *const _ as *mut u8 as *mut T
         } else {
@@ -357,8 +367,6 @@ impl<T: IntoPyObject> ReturnTypeIntoPyResult for PyResult<T> {
     }
 }
 
-
-
 #[cfg(test)]
 mod test {
     use super::PyTryFrom;
diff --git a/src/instance.rs b/src/instance.rs
index d5fd4995..d5ab353f 100644
--- a/src/instance.rs
+++ b/src/instance.rs
@@ -55,6 +55,15 @@ impl<'a, T> Deref for PyRef<'a, T> {
     }
 }
 
+impl<'a, T> Into<&'a PyObjectRef> for PyRef<'a, T>
+where
+    T: PyTypeInfo,
+{
+    fn into(self) -> &'a PyObjectRef {
+        unsafe { &*(self.as_ptr() as *const PyObjectRef) }
+    }
+}
+
 #[derive(Debug)]
 pub struct PyRefMut<'a, T> {
     inner: &'a mut T,
@@ -91,18 +100,27 @@ impl<'a, T> DerefMut for PyRefMut<'a, T> {
     }
 }
 
+impl<'a, T> Into<&'a PyObjectRef> for PyRefMut<'a, T>
+where
+    T: PyTypeInfo,
+{
+    fn into(self) -> &'a PyObjectRef {
+        unsafe { &*(self.as_ptr() as *const PyObjectRef) }
+    }
+}
+
 /// Trait implements object reference extraction from python managed pointer.
 pub trait AsPyRef<T>: Sized {
     /// Return reference to object.
-    fn as_ref(&self, py: Python) -> &T;
+    fn as_ref(&self, py: Python) -> PyRef<T>;
 
     /// Return mutable reference to object.
-    fn as_mut(&mut self, py: Python) -> &mut T;
+    fn as_mut(&mut self, py: Python) -> PyRefMut<T>;
 
     /// Acquire python gil and call closure with object reference.
     fn with<F, R>(&self, f: F) -> R
     where
-        F: FnOnce(Python, &T) -> R,
+        F: FnOnce(Python, PyRef<T>) -> R,
     {
         let gil = Python::acquire_gil();
         let py = gil.python();
@@ -113,7 +131,7 @@ pub trait AsPyRef<T>: Sized {
     /// Acquire python gil and call closure with mutable object reference.
     fn with_mut<F, R>(&mut self, f: F) -> R
     where
-        F: FnOnce(Python, &mut T) -> R,
+        F: FnOnce(Python, PyRefMut<T>) -> R,
     {
         let gil = Python::acquire_gil();
         let py = gil.python();
@@ -124,7 +142,7 @@ pub trait AsPyRef<T>: Sized {
     fn into_py<F, R>(self, f: F) -> R
     where
         Self: IntoPyPointer,
-        F: FnOnce(Python, &T) -> R,
+        F: FnOnce(Python, PyRef<T>) -> R,
     {
         let gil = Python::acquire_gil();
         let py = gil.python();
@@ -137,7 +155,7 @@ pub trait AsPyRef<T>: Sized {
     fn into_mut_py<F, R>(mut self, f: F) -> R
     where
         Self: IntoPyPointer,
-        F: FnOnce(Python, &mut T) -> R,
+        F: FnOnce(Python, PyRefMut<T>) -> R,
     {
         let gil = Python::acquire_gil();
         let py = gil.python();
@@ -307,12 +325,12 @@ where
     T: PyTypeInfo,
 {
     #[inline]
-    fn as_ref(&self, py: Python) -> &T {
-        self.as_ref_dispatch(py)
+    fn as_ref(&self, py: Python) -> PyRef<T> {
+        PyRef::new(self.as_ref_dispatch(py))
     }
     #[inline]
-    fn as_mut(&mut self, py: Python) -> &mut T {
-        self.as_mut_dispatch(py)
+    fn as_mut(&mut self, py: Python) -> PyRefMut<T> {
+        PyRefMut::new(self.as_mut_dispatch(py))
     }
 }
 
diff --git a/src/object.rs b/src/object.rs
index cfd7c771..dbac3955 100644
--- a/src/object.rs
+++ b/src/object.rs
@@ -7,7 +7,7 @@ use crate::conversion::{
 };
 use crate::err::{PyDowncastError, PyErr, PyResult};
 use crate::ffi;
-use crate::instance::{AsPyRef, PyObjectWithGIL};
+use crate::instance::{AsPyRef, PyObjectWithGIL, PyRef, PyRefMut};
 use crate::python::{IntoPyPointer, Python, ToPyPointer};
 use crate::pythonrun;
 use crate::types::{PyDict, PyObjectRef, PyTuple};
@@ -154,7 +154,7 @@ impl PyObject {
     /// Casts the PyObject to a concrete Python object type.
     pub fn cast_as<D>(&self, py: Python) -> Result<&D, PyDowncastError>
     where
-        D: PyTryFrom,
+        D: for<'v> PyTryFrom<'v>,
     {
         D::try_from(self.as_ref(py))
     }
@@ -165,7 +165,7 @@ impl PyObject {
     where
         D: FromPyObject<'p>,
     {
-        FromPyObject::extract(self.as_ref(py))
+        FromPyObject::extract(self.as_ref(py).into())
     }
 
     /// Retrieves an attribute value.
@@ -256,12 +256,12 @@ impl PyObject {
 
 impl AsPyRef<PyObjectRef> for PyObject {
     #[inline]
-    fn as_ref(&self, _py: Python) -> &PyObjectRef {
-        unsafe { &*(self as *const _ as *const PyObjectRef) }
+    fn as_ref(&self, _py: Python) -> PyRef<PyObjectRef> {
+        unsafe { PyRef::new(&*(self as *const _ as *const PyObjectRef)) }
     }
     #[inline]
-    fn as_mut(&mut self, _py: Python) -> &mut PyObjectRef {
-        unsafe { &mut *(self as *mut _ as *mut PyObjectRef) }
+    fn as_mut(&mut self, _py: Python) -> PyRefMut<PyObjectRef> {
+        unsafe { PyRefMut::new(&mut *(self as *mut _ as *mut PyObjectRef)) }
     }
 }
 
diff --git a/src/objectprotocol.rs b/src/objectprotocol.rs
index a4bf2372..e3c20052 100644
--- a/src/objectprotocol.rs
+++ b/src/objectprotocol.rs
@@ -187,7 +187,7 @@ pub trait ObjectProtocol {
     /// Casts the PyObject to a concrete Python object type.
     fn cast_as<'a, D>(&'a self) -> Result<&'a D, PyDowncastError>
     where
-        D: PyTryFrom,
+        D: PyTryFrom<'a>,
         &'a PyObjectRef: std::convert::From<&'a Self>;
 
     /// Extracts some type from the Python object.
@@ -471,10 +471,10 @@ where
 
     fn cast_as<'a, D>(&'a self) -> Result<&'a D, PyDowncastError>
     where
-        D: PyTryFrom,
+        D: PyTryFrom<'a>,
         &'a PyObjectRef: std::convert::From<&'a Self>,
     {
-        D::try_from(self.into())
+        D::try_from(self)
     }
 
     fn extract<'a, D>(&'a self) -> PyResult<D>
diff --git a/src/types/iterator.rs b/src/types/iterator.rs
index 0a74dfe8..70943658 100644
--- a/src/types/iterator.rs
+++ b/src/types/iterator.rs
@@ -85,12 +85,12 @@ impl<'p> Drop for PyIterator<'p> {
 mod tests {
     use indoc::indoc;
 
-    use crate::conversion::{PyTryFrom, ToPyObject};
+    use crate::conversion::ToPyObject;
     use crate::instance::AsPyRef;
     use crate::objectprotocol::ObjectProtocol;
     use crate::python::Python;
     use crate::pythonrun::GILPool;
-    use crate::types::{PyDict, PyList, PyObjectRef};
+    use crate::types::{PyDict, PyList};
     use crate::GILGuard;
 
     #[test]
@@ -98,7 +98,7 @@ mod tests {
         let gil_guard = Python::acquire_gil();
         let py = gil_guard.python();
         let obj = vec![10, 20].to_object(py);
-        let inst = <PyObjectRef as PyTryFrom>::try_from(obj.as_ref(py)).unwrap();
+        let inst = obj.as_ref(py);
         let mut it = inst.iter().unwrap();
         assert_eq!(10, it.next().unwrap().unwrap().extract().unwrap());
         assert_eq!(20, it.next().unwrap().unwrap().extract().unwrap());
@@ -119,7 +119,7 @@ mod tests {
         {
             let gil_guard = Python::acquire_gil();
             let py = gil_guard.python();
-            let inst = <PyObjectRef as PyTryFrom>::try_from(obj.as_ref(py)).unwrap();
+            let inst = obj.as_ref(py);
             let mut it = inst.iter().unwrap();
 
             assert_eq!(10, it.next().unwrap().unwrap().extract().unwrap());
@@ -147,7 +147,7 @@ mod tests {
 
         {
             let _pool = GILPool::new();
-            let inst = <PyObjectRef as PyTryFrom>::try_from(obj.as_ref(py)).unwrap();
+            let inst = obj.as_ref(py);
             let mut it = inst.iter().unwrap();
 
             assert_eq!(10, it.next().unwrap().unwrap().extract().unwrap());
diff --git a/src/types/sequence.rs b/src/types/sequence.rs
index 8127ed01..7cadfa7b 100644
--- a/src/types/sequence.rs
+++ b/src/types/sequence.rs
@@ -275,8 +275,9 @@ where
     Ok(v)
 }
 
-impl PyTryFrom for PySequence {
-    fn try_from(value: &PyObjectRef) -> Result<&PySequence, PyDowncastError> {
+impl<'v> PyTryFrom<'v> for PySequence {
+    fn try_from<V: Into<&'v PyObjectRef>>(value: V) -> Result<&'v PySequence, PyDowncastError> {
+        let value = value.into();
         unsafe {
             if ffi::PySequence_Check(value.as_ptr()) != 0 {
                 Ok(<PySequence as PyTryFrom>::try_from_unchecked(value))
@@ -286,11 +287,16 @@ impl PyTryFrom for PySequence {
         }
     }
 
-    fn try_from_exact(value: &PyObjectRef) -> Result<&PySequence, PyDowncastError> {
+    fn try_from_exact<V: Into<&'v PyObjectRef>>(
+        value: V,
+    ) -> Result<&'v PySequence, PyDowncastError> {
         <PySequence as PyTryFrom>::try_from(value)
     }
 
-    fn try_from_mut(value: &PyObjectRef) -> Result<&mut PySequence, PyDowncastError> {
+    fn try_from_mut<V: Into<&'v PyObjectRef>>(
+        value: V,
+    ) -> Result<&'v mut PySequence, PyDowncastError> {
+        let value = value.into();
         unsafe {
             if ffi::PySequence_Check(value.as_ptr()) != 0 {
                 Ok(<PySequence as PyTryFrom>::try_from_mut_unchecked(value))
@@ -300,19 +306,21 @@ impl PyTryFrom for PySequence {
         }
     }
 
-    fn try_from_mut_exact(value: &PyObjectRef) -> Result<&mut PySequence, PyDowncastError> {
+    fn try_from_mut_exact<V: Into<&'v PyObjectRef>>(
+        value: V,
+    ) -> Result<&'v mut PySequence, PyDowncastError> {
         <PySequence as PyTryFrom>::try_from_mut(value)
     }
 
     #[inline]
-    unsafe fn try_from_unchecked(value: &PyObjectRef) -> &PySequence {
-        let ptr = value as *const _ as *const PySequence;
+    unsafe fn try_from_unchecked<V: Into<&'v PyObjectRef>>(value: V) -> &'v PySequence {
+        let ptr = value.into() as *const _ as *const PySequence;
         &*ptr
     }
 
     #[inline]
-    unsafe fn try_from_mut_unchecked(value: &PyObjectRef) -> &mut PySequence {
-        let ptr = value as *const _ as *mut PySequence;
+    unsafe fn try_from_mut_unchecked<V: Into<&'v PyObjectRef>>(value: V) -> &'v mut PySequence {
+        let ptr = value.into() as *const _ as *mut PySequence;
         &mut *ptr
     }
 }
diff --git a/src/types/string.rs b/src/types/string.rs
index 73d10348..5a8b7663 100644
--- a/src/types/string.rs
+++ b/src/types/string.rs
@@ -141,7 +141,7 @@ mod test {
         let s = "Hello Python";
         let py_string = s.to_object(py);
 
-        let s2: &str = FromPyObject::extract(py_string.as_ref(py)).unwrap();
+        let s2: &str = FromPyObject::extract(py_string.as_ref(py).into()).unwrap();
         assert_eq!(s, s2);
     }
 
diff --git a/src/types/tuple.rs b/src/types/tuple.rs
index afd38304..6a3e7c04 100644
--- a/src/types/tuple.rs
+++ b/src/types/tuple.rs
@@ -115,7 +115,7 @@ impl<'a> Iterator for PyTupleIterator<'a> {
         if self.index < self.slice.len() {
             let item = self.slice[self.index].as_ref(self.py);
             self.index += 1;
-            Some(item)
+            Some(item.into())
         } else {
             None
         }